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Harmonic Nanoparticles for R&D Activities
A Tibio product. Tel: +41 91 940 63 31 Fax: +41 91 940 63 33hnp@tibio.ch

Pagina Principale, Inglese

Overview

Harmonic nanoparticles (HNP) are optically active nanoparticles exhibiting a strong and selective response to nonlinear optical excitation. They represent a novel family of inherently nonlinear, non-fluorescent nanoparticles, specifically conceived and realized for second harmonic generation (SHG) and third harmonic generation (THG) [1,2,3]. HNP are based on inorganic crystals characterized by a noncentrosymmetric structure, by which they possess the SHG response [1,2]. HNP have been recently promoted to address some specific issues related to bio-imaging performances of fluorescent markers, since they displayed a series of interesting optical properties never obtained before in the same optical probe and outperform fluorescent and luminescent labels with respect to [1]: 1. Signal stability: no bleaching and blinking. 2. Hindrance by autofluorescence: HNP do not present any specific wavelength dependence, thus the excitation wavelength can be freely tuned over range from UV to infra-red. 3. Signal saturation: not present. Moreover, more refined techniques can be applied for specific applications, i.e. polarization sensitive response and coherence [4,5,6]. HNP for bio-medical applications: recent studies have shown that HNP are characterized by a good biocompatibility and displayed interesting characteristics for therapeutic applications, such as phototherapy [3,7,8]. Typical applications of HNP: – Cell and tissue labellingAssessment of non-linear microscopy performanceOn-demand frequency generationDetection in fluids We supply three different uncoated and dispersed nanocrystals composed by barium titanate (BaTiO3), bismuth ferrite (BiFeO3, BFO) and potassium niobate (KNbO3). These HNP are useful for optical, in-vitro and ex-vivo experiments and are available in 1 mL vials at 5 mg/mL in 100% ethanol. This quantity is sufficient for approximately 100 to 400 experiments.
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(1) Human derived cells stained with a fluorescent probe for lipid membranes (red) and exposed to harmonic nanoparticles (blue). (2)(3) Human derived cells stained with a fluorescent probe for lipid membranes (yellow) and exposed to harmonic nanoparticles (blue). (4) Interference pattern generated by simultaneously exciting the second harmonic of two neighboring harmonic nanoparticles.
 

Barium titanate (BaTiO3) HNPs for R&D Barium titanate (BaTiO3) HNP are available in 1 mL vials at 5 mg/mL in 100% ethanol.  
Current lot available (Lot number): Not yet available
Concentration: -.- mg/mL
Zeta-potential: -.- mV
Average size (DLS*): —.- nm
Size distribution (DLS)*
Optical characteristics
Normalized hyperpolarizability[3] 4.6 + 0.7 pm/V
Symmetry class 4mm
* Dynamic light scattering IMPORTANT: disperse the HNP by immerse the vials in an ultrasonic bath (45 kHz) at room temperature for 30 min before use. Storage: at room temperature with protection from light.

Bismuth ferrite (BiFeO3) HNPs for R&D Bismuth ferrite (BiFeO3) HNP are available in 1 mL vials at 5 mg/mL in 100% ethanol. Selling price: 1 box with 1x 1 mL vial: 175.- EUR (for approximately 100 to 400 experiments – VAT and shipping costs exluded) 1 box with 2x 1 mL vials: 305.- EUR (for approximately 200 to 800 experiments – VAT and shipping costs exluded)    
Current lot available (Lot number): BF0011214 (from January 5th 2015)
Concentration: 5.0 mg/mL
Zeta-potential: -22.9 + 0.5 mV
Average size (DLS*): 122.4 + 0E+00 nm
Size distribution (DLS)*
Optical characteristics
Normalized hyperpolarizability[6] 79.0 + 11.8 pm/V
Symmetry class R3c
* Dynamic light scattering IMPORTANT: disperse the HNP by immerse the vials in an ultrasonic bath (45 kHz) at room temperature for 30 min before use. Storage: at room temperature with protection from light.

Potassium niobate (KNbO3) HNPs for R&D Potassium niobate (KNbO3) HNP are available in 1 mL vials at 5 mg/mL in 100% ethanol. Selling price: 1 box with 1x 1 mL vial: 135.- EUR (for approximately 100 to 400 experiments – VAT and shipping costs exluded) 1 box with 2x 1 mL vials: 230.- EUR (for approximately 200 to 800 experiments – VAT and shipping costs exluded)  
Current lot available (Lot number): KB0011014 (from November 20th 2014)
Concentration: 5.0 mg/mL
Zeta-potential: -23.6 + 1.2 mV
Average size (DLS*): 130.8 + 30.1 nm
Size distribution (DLS)*
Optical characteristics
Normalized hyperpolarizability[3] 3.4 + 1.1 pm/V
Symmetry class mm2
* Dynamic light scattering IMPORTANT: disperse the HNP by immerse the vials in an ultrasonic bath (45 kHz) at room temperature for 30 min before use. Storage: at room temperature with protection from light.

Order

You may place your orders by email: hnp@tibio.ch Indicating your shipping and billing address, what type of HNP do you want and how many vials. Currently we accept payment only by wire transfer. All transactions must be completed in Euro (EUR). Payment policy: NET 30 days. Advance payment is asked for orders of 1’000.- EUR or more. Orders typically arrive within 5-10 days, depending on product availability. Shipping cost: Ask our customer service: hnp@tibio.ch

References and Links

[1] Bonacina L. Nonlinear nanomedecine: harmonic nanoparticles toward targeted diagnosis and therapy. Mol Pharm 2013; 10, 783.
[2] Geissbuehler, M., Bonacina, L., Shcheslavskiy, V., et al. Nonlinear Correlation Spectroscopy (NLCS). Nano Lett 2012; 12, 1668. [3] Staedler D, Magouroux T, Hadji R, et al. Harmonic nanocrystals for biolabeling: a survey of optical properties and biocompatibility. ACS Nano 2012; 6, 2542. [4] Bonacina, L., Mugnier, Y., Courvoisier, F., et al. Polar Fe(IO3)(3) nanocrystals as local probes for nonlinear microscopy. Appl Phys B-Lasers O 2007; 87, 399. [5] Extermann, J., Bonacina, L., Courvoisier, F., et al. Nano-FROG: Frequency resolved optical gating by a nanometric object. Opt Express 2008; 16, 10405. [6] Schwung, S., Rogov, A., Clarke, G., et al. Nonlinear optical and magnetic properties of BiFeO3 harmonic nanoparticles. J Appl Phys 2014; 116, 114306. [7] Magouroux T, Extermann J, Hoffmann P, et al. High-speed tracking of murine cardiac stem cells by harmonic nanodoublers. Small 2012; 8, 2752. [8] Staedler D, Magouroux T, Passemard S, et al. Deep UV generation and direct DNA photo-interaction by harmonic nanoparticles in labelled samples. Nanoscale 2014; 6, 2929.
Development partially supported by the European Commission FP7 NAMDIATREAM project (EU NMP4-LA-2010-246479): www.namdiatream.eu Images credits: www.gap.unige.ch/biophotonics A product by: www.tibio.ch Website: www.no-plans.com